pi :: Psychiatry Investigation

Moon, Lee, and Choi: Changes in the Hippocampal Volume and Shape in Early-Onset Mild Cognitive Impairment

Original Article

Psychiatry Investigation 2018;15(5):531-537.

Published online: April 27, 2018

DOI: https://doi.org/10.30773/pi.2018.02.12

Changes in the Hippocampal Volume and Shape in Early-Onset Mild Cognitive Impairment

Seok Woo Moon¹, Boram Lee², Young Chil Choi³

¹Department of Psychiatry, Konkuk University School of Medicine, Chungju, Republic of Korea

²Department of Psychiatry, Graduate School of Konkuk University, Seoul, Republic of Korea

³Department of Radiology, Konkuk University School of Medicine, Chungju, Republic of Korea

Correspondence: Seok Woo Moon, MD, PhD Department of Psychiatry, Konkuk University Chungju Hospital, 82 Gukwondaero, Chungju 27354, Republic of Korea Tel: +82-43-840-8990, Fax: +82-43-857-1380, E-mail: hessem@nate.com

Received November 30, 2017 Revised January 23, 2018 Accepted February 12, 2018

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective

The aim of this study was to examine the change in the hippocampal volume and shape in early-onset mild cognitive impairment (EO-MCI) associated with the APOE ε4 carrier state.

Methods

This study had 50 subjects aged 55–63 years, all of whom were diagnosed with MCI at baseline via the Korean version of the Consortium to Establish a Registry for Alzheimer’s Disease Assessment Packet. The EO-MCI patients were divided into the MCI continued (MCIcont) and Alzheimer’s disease (AD) converted (ADconv) groups 2 years later. The hippocampal volume and shape were measured for all the subjects. The local shape analysis (LSA) was used to conduct based on the 2-year-interval magnetic resonance imaging scans.

Results

There was a significant correlation between APOE ε4 allele and hippocampal volume atrophy. Over two years, the volume reduction in the left hippocampus was found to be faster than that in the right hippocampus, especially in the APOE ε4 carriers. LSA showed that the 2 subfields were significantly affected in the left hippocampus.

Conclusion

These results suggest that the possession of APOE ε4 allele may lead to greater predilection for left hippocampal atrophy in EO-MCI, and some specific subfields of the hippocampus may be more prominently involved.

Keywords: Early onset, Mild cognitive impairment, Hippocampus, APOE ε4, Atrophy

INTRODUCTION

Mild cognitive impairment (MCI), especially the amnestic type of MCI, refers to a prodromal stage of Alzheimer’s disease (AD) during which patients display cognitive impairment without apparent disabilities in their activities of daily living. Patients with MCI show memory dysfunction, and 10–20% of MCI annually progresses into AD [1,2]. Atrophy of the hippocampus is one of the most sensitive biological indicators of AD, and hippocampal volumetry is the best-established structural biomarker for AD, especially for early diagnosis [3-8]. Earlier studies have reported that hippocampal atrophy can to some extent predict which patients with MCI will subsequently convert to AD [9,10], and that the AD converters (ADconv) group had an enhanced rate of hippocampal volume loss [11]. The ADconv group had a significantly higher rate of hippocampal volume loss compared to the MCI continued (MCIcont) group [12,13].

There are two main subtypes of AD: early-onset AD (EO-AD) and sporadic AD. A very small percentage of the people who develop AD have the EO variety, which is classified as the beginning of the disease presentation before the age of 65. Researchers have discovered three genes in which mutations cause EO-AD [14]. These known genetic mutations, however, account for only 2% of all the cases of EO-AD [15]. The other group, sporadic AD, is most commonly termed “late-onset AD (LO-AD).” It is defined by the disease presentation after the age of 65, and it is well known that the apolipoprotein E (APOE) ε4 allele can influence it. According to the current viewpoint, however, classifying AD into EO or LO is probably not useful from a mechanistic point of view because mutations in the 3 genes can be found in both EO and LO. Similarly, APOE ε4 allele increases the risk of AD in both EO-AD and LO-AD. Accordingly, EO-AD that does not have an autosomal dominant pattern can share similar characteristics with LO-AD and may be influenced by the APOE ε4 allele, similar to LO-AD [14,16]. Considering that MCI is a transitional state between normal aging and AD, it may be speculated that MCI can also be divided into EO-MCI and LO-MCI according to the age of symptom onset. EO-MCI and LO-MCI may be expected to have patterns of differences similar to those between EO-AD and LO-AD because EO-AD and LO-AD can have different clinical features and anatomical substrates [17].

The identification and characterization of patients with EOMCI may have clinical significance because the potential impact of cognitive impairment on vocational and social activities is probably greater in the young than in the older MCI patients [18]. To the authors’ knowledge, however, there has been no study exploring EO-MCI and comparing it with LO-MCI in terms of the imaging characteristics.

Recent research has shown that the APOE ε4 allele is well known as the risk factor not only for LO-AD but also for EOAD [14,16,19]. Studies involving EO-AD subjects under 65 years associated with the APOE ε4 allele are not common [17], and the research on EO-MCI is still limited because all the studies so far have focused on LO-MCI/AD, which is more common than EO-MCI/AD [20]. Additionally, the hippocampus is not a homogeneous structure but consists of several subfields with distinct histological characteristics: the subiculum, the three cornu ammonis (CA) sectors (CA1, CA2, and CA3), and the dentate gyrus [21]. Therefore, this neuroimaging study is expected to explain some of the characteristics of the MCIcont and ADconv groups in terms of volume- and shape- based morphometrics in the EO-MCI subjects using hippocampal volumetry and local shape analysis (LSA) [7,22]. The authors targeted EO-MCI subjects under 65 years who had very important diagnostic values, divided them into two groups according to their APOE ε4 status, and checked their brain magnetic resonance imaging (MRI) scans at baseline and 2 year later. This study was done to elucidate the changes in the hippocampal volume and shape in the EO-MCI subjects according to their APOE ε4 status and according to their AD dementia conversion.

METHODS

Participants

The study subjects were selected while in the implementation of the dementia early screening project of the Ministry of Health and Welfare from March 2008 to February 2016. A skilled geropsychiatrist and trained research nurses performed examinations with the Korean version of the Consortium to Establish a Registry for Alzheimer’s Disease Assessment Packet (CERAD-K) for the elderly aged 55–63 years who were residing in the Chungbuk area, and those who were finally diagnosed with amnestic MCI at baseline were targeted for the study. Of them, those who had a Clinical Dementia Rating (CDR) score of 0.5 in the examination, who did not have a history of cerebrovascular disease, who did not have a major depressive disorder based on Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition, Text Revision (DSM-IV-TR), and who had a Modified Hachinski Ischemic Scale [23] score of 3 or less were included in the study. On the other hand, those with delirium, confusion, or other clouded consciousness; those with neurological diseases such as Parkinson’s disease, stroke, tumors, and normal pressure hydrocephalus; those with evidence of severe cerebrovascular disease; and those who had medical conditions that might have serious effects on their cognitive functions or who were taking drugs affecting their cognitive functions were excluded. A total of 66 persons at baseline were selected as study subjects and gave their consent for genetic testing to check the presence of the APOE ε4 allele. With the subjects who did not undergo genetic testing being excluded, a total of 53 study subjects were selected. All the study subjects went through brain MRI scan at baseline and 2 years later. As a result, based on the brain MRI scan, a total of 50 subjects were included in this study: 35 subjects who met the aforementioned diagnosis criteria and were diagnosed with MCI (MCIcont), and 15 subjects who were diagnosed with AD dementia (ADconv). Meanwhile, three subjects were excluded. Of these 3 subjects, two subjects were excluded due to vascular dementia, and one subject was excluded due to frontotemporal dementia. Written informed consent was obtained from each patient, and this study was reviewed and approved by the local IRB (KUCH 2017-12-033).

Imaging protocol

Brain MRI acquisition

Brain MRI scan was conducted at the Department of Radiology of a university hospital, using a 1.5-Tesla scanner (MRT200PP3, Toshiba, Japan). With the following parameters, the MRI scans were obtained: repetition time (TR)=15 ms, echo time (TE)=5 ms, field of view (FOV)=240×240 mm, flip angle=20°, slice thickness=1.5 mm, matrix size=256×256, acquisition time=3.06 min, resolution=0.93×0.93×1.5 mm, yielding 3D T1-weighted images.

Hippocampal volume and atrophy rate measurements

The hippocampus was manually labeled by a rater in the contiguous coronal brain sections using BrainSuite24 and guided by well-established protocols (http://users.loni.ucla.edu/~narr/protocol.php?q=hippotrace). The same rater repeated this process three times. After manually labeling the hippocampus as described above, the global left and right hippocampal volumes were established (in mm³) based on the number of voxels constituting the hippocampus labels.

To compare the baseline hippocampal volume (i.e., hippocampal volume at the time of the initial MRI scan) of the study groups, the hippocampal volume measurements were normalized for inter-individual variation in head size, age, and gender [25]. The hippocampal volumes in each individual were summed and divided by the total intracranial volume (ICV) of that particular patient to control for variation in head size [12]. ICV measurement was done automatically using the Lab of NeuroImaging (LONI) pipeline system of University of Southern California (USC) [26]. The hippocampal atrophy rates were calculated based on the reduction percentages of the hippocampal volumes between the 2-year-interval MRI scans.

Local shape analysis (LSA)

The LSA pipeline workflow was used to conduct local (pervertex) post-hoc statistical analyses of the shape differences between the two-year-interval brain MRI scans in the left and right hippocampus. In the LSA pipeline workflow, the 3D structural MRI data are first preprocessed (skull-stripped, spatially normalized, parcellated) [26,27], then shape models of the hipocampi are generated as genus-zero two-dimensional manifolds [28,29]. By traversing the triangulated boundary manifolds (per-vertex), statistical significance maps representing the group differences in two complementary shape metrics are obtained. The radial distance measures at each vertex encode the magnitude and direction of the local shape morphometry, which quantify the discrepancy between each subject as the “mean shape” (boundary) for both hippocampi. Probability values corresponding to the test statistics are overlaid on the mean boundary shape for each region to illustrate the group differences.

Statistical analyses

Group differences between the MCIcont and ADconv groups were analyzed by Student’s t-test for continuous variables such as age or education years or χ² (chi-squared) test for categorical variables such as the gender and the APOE ε4 carrier status. According to the presence of ε4, both hippocampi volumes were analyzed with a 2-year interval via repeated-measures ANOVA (analysis of variance), controlling the age and gender, which might affect the results. In addition, for group comparisons, the unpaired t-test (two-tailed) was used together with Mann-Whitney U test as a non-parametric statistical analysis. Effects of the age, gender, and APOE ε4 allele on the atrophy rate of both hippocampi were measured using general linear model (GLM) analyses for the MCIcont and ADconv groups respectively. For the analysis, SPSS version 20.0 for the Windows program was used.

Moreover, the statistical method of the multiple linear regression (MLR) model was used to conduct the LSA analysis, and a design matrix that included age, gender, and APOE ε4 status as regressors was used. The EO-MCI subjects were analyzed as a whole and by group (MCIcont and ADconv groups), using the anatomical morphometric measures as predictors of the diagnosis as the response variables. For LSA to analyze the shape, the MLR model was applied using the Laboratory of NeuroImaging (LONI) pipeline system of USC.

RESULTS

Demographic and clinical characteristics of the subjects

Among the total of 50 study subjects, 35 (70%) were included in MCIcont group and 15 (30%) were included in the ADconv group 2 years later. The mean age was 63.81±3.19 (M±SD) years in the MCIcont group and 63.64±3.01 (M±SD) years in the ADconv group, not showing a significant difference (t=0.149, p=0.883). The mean education years was not showing a significant difference (t=0.276, p=0.785). The MMSEKC score was 25.25±1.43 (M±SD) in the MCIcont group and 20.24±3.12 (M±SD) in the ADconv group, showing a significant difference (t=1.134, p=0.001). The APOE ε4 status was not showing a significant difference (χ²=0.736, p=0.391). The demographic and clinical data are described in Table 1.

Changes in the hippocampal volume over 2 years in the MCIcont and ADconv groups

As a result of the re-measurement of the hippocampal volume with a 2-year interval from the initial measurement at the baseline, there was a significant change in the volume regardless of the presence of APOE ε4 in both the MCIcont and ADconv groups. In the case of the left hippocampus, a more statistically significant result was shown in the ADconv group especially when APOE ε4 allele was present (F=11.470, p=0.001) (Table 2).

Differences in atrophy rates between the MCIcont and ADconv groups with regard to the APOE ε4 status

In the case of the left hippocampus, the atrophy rate was significantly higher in the ADconv group than in the MCIcont group within the APOE ε4 carrier group (t=-2.91, p=0.005, tied p=0.003). Even within the ADconv group, the atrophy rate was significantly higher in the APOE ε4 carrier group than in the APOE ε4 non-carrier group (t=-2.58, p=0.016, tied p=0.010). In the case of the right hippocampus, the atrophy rate tended to be higher but it was not statistically significant (Table 3).

Effect of APOE ε4 on the atrophy rate of both hippocampi in the MCIcont group: results of the GLM analysis

In the MCIcont group, the atrophy rate of the left hippocampus was higher in the APOE ε4 carrier group in the GLM analysis, regardless of the age and gender (F=5.532, p=0.025). The atrophy rate of the right hippocampus tended to be higher in the APOE ε4 carrier group, but it was not statistically significant (F=3.82, p=0.056) (Table 4).

Effect of APOE ε4 on the atrophy rate of both hippocampi in the ADconv group: results of the GLM analysis

In the ADconv group, the atrophy rate of the left hippocampus was higher in the APOE ε4 carrier group in the GLM analysis, regardless of the age and gender (F=12.978, p=0.001). Even the atrophy rate of the right hippocampus was still significantly higher in the APOE ε4 carrier group (F=11.05, p=0.007) (Table 4).

Local shape analysis

Both hippocampi were chosen to find out if their shape measures are also associated with the changes in the 2-year-interval MRI scans (regressor). The result of the LSA of the hippocampus in the MRI scanning with a 2-year interval showed no significant outcome surviving the false discovery rate (FDR) correction in the analysis of the difference between the MCIcont and ADconv groups and in the analysis based on the presence of APOE ε4. Only when the MRI values of the total EO-MCI group were compared without controlling any variable with a 2-year interval was it found that the shape of the left hippocampus was significantly changed in the hippocampal radial distance and survived the FDR correction. In the case of the right hippocampus, however, no case survived the FDR correction. All the results that survived the FDR correction are shown as p-value color maps in Figure 1. Figure illustrates the significant atrophic change in the hippocampal radial distance between the 2-year follow-up scans at the left hippocampus.

DISCUSSION

The main findings in this study were that the hippocampal atrophy rates predict conversion to dementia in MCI patients, and that the left hippocampal volume associated with the APOE ε4 allele seems to be the best marker for the ADconv group in EO-MCI. In this study, the atrophy rate of the left hippocampus was significantly higher than the right hippocampus in the APOE ε4 carriers of the MCIcont group. The atrophy rate of both hippocampi in the ADconv group was significantly higher in the APOE ε4 carriers than in the APOE ε4 non-carriers, but the atrophy rate of the left hippocampus was higher than the atrophy rate of the right hippocampus. In LSA for hippocampal shape measure, the radial distance atrophy in the left hippocampus was significantly changed regardless of APOE ε4 and the AD conversion status. A previous 3-year clinical follow-up LO study reported that the mean annualized rates of hippocampal atrophy were control, 1.73%; MCIcont, 2.55%; ADconv, 3.69%; and AD, 3.5%. The main findings of the study were that the hippocampal volume predicts conversion to dementia in LO-MCI patients, and that the left hippocampal volume seems to be the best marker for conversion [12]. Another LO study reported that the mean annualized rates of hippocampal atrophy were MCIcont, 3.0% and ADconv, 4.1% [30]. Taken together from the LO studies, including the two aforementioned studies, the volume of the hippocampus is reduced by 1.55–2.09% annually in normal persons in general, while it is reduced by 2.55–3.0% annually in the MCIcont group and 3.69–4.1% annually in the ADconv group [31]. Thus, if the volume of the hippocampus is reduced by more than 3.0% annually in LO-MCI patients, the possibility of developing AD dementia is relatively higher. In this EO study, it was found that the annualized rates of hippocampal atrophy by 2-year follow-up were MCIcont, 2.38% and ADconv, 2.79%. This EO study on hippocampal atrophy rates revealed that the rates were lower than those in the previous LO studies. This difference can be explained from a previous topographic study which indicate that EO-AD and LO-AD differ in their typical topographic patterns of brain atrophy, suggesting different predisposing or etiological factors [19]. Given that EO-AD and LO-AD have different clinical features and anatomical substrates, EO-MCI and LO-MCI may be expected to have patterns of differences similar to those between EO-AD and LO-AD [17]. As one more reason for slow atrophy in EO-MCI, it can be said that EO-MCI is a less characterized group and is probably more heterogeneous than LO-MCI, including more patients with other comorbidities [32,33]. Therefore, the relatively lower prevalence of AD among people under 65 years of age, and the higher prevalence of other types of dementia [18], can lead to slower atrophy in EO-MCI than in LO-MCI [34]. These reports may say that the hippocampal atrophy can be less influenced in EO-MCI/AD than in LO-MCI/AD. It is difficult to conclusively say, however, that the hippocampal volume atrophy is trivial because the atrophy in EO-AD/MCI is slow. EO-MCI/AD may first affect another area (including neocortex) but will affect the hippocampus as well, on the extension of LO-MCI/AD, and its atrophy can be still important in predicting AD dementia conversion. This study showed that the degree of hippocampal atrophy of EO-MCI does not seem to be larger than that of LO-MCI, but it can still be higher associated with the moderating effect of the APOE ε4 allele. Even though the hippocampal atrophy is known to be smaller in EOMCI/AD than in LO-MCI/AD, EO and LO can be considered as one continuum since the age of 65 is an arbitrary cut-off, which does not have a true biological meaning [35,36].

In terms of our data, annual EO-AD conversion rate was 15% (30% for 2 years) and was close to the conversion rate in LO-MCI, which can say that EO-MCI and LO-MCI may lie in a continuum. On the other hand, when it comes to hippocampal atrophy rate, it was slower in EO-MCI than in LO-MCI, which can show us that the atrophy in hippocampus, is less influenced in EO-MCI.

In line with volume-based comparison, our shape-based diffeomorphometric comparison results of LSA revealed that radial distance atrophy in left hippocampus was significantly changed. The radial distance measures the distance from each surface point to the central core of the shape (point of gravitational balance). This metric index captures local expansions or contractions of the developing cortical surface. Previous research shows that the hippocampal formation is unevenly affected by AD pathology, deposits of plaques and tangles being particularly dense in the CA1 field and subiculum [3], and regions corresponding to the CA2/3 fields were remarkably spared [37,38]. Our data are very consistent with the results of this research on hippocampal atrophy rates and associated with previous morphometric comparison research on hippocampus as well.

This study associated with EO-MCI, especially with ADconv group, revealed that faster left hippocampal atrophy in volume especially in APOE ε4 carriers. Similarly, Risacher et al. [39] reported that decreased hippocampal volume (left more than right) was the most robust structural MRI feature. Our study can reveal that left hippocampus is more atrophied in EO-MCI, as a continuum in LO-MCI.

In this study, the moderating effect of the APOE ε4 allele on hippocampal atrophy was shown in both hippocampi atrophy rates, but the moderating effect for the left hippocampus was more remarkable. Several studies reported that the left hippocampus related or not related to the APOE ε4 allele contracts significantly faster in LO-MCI [11,39,40]. This EO-MCI study including LSA for the hippocampus showed the same result. Therefore, volume- and shape- based research should be continuously performed for the issue that the moderating effect of the APOE ε4 allele on the left hippocampus affects the conversion into AD dementia.

There are several limitations to this EO study. First, the sample size was small due to the significant data stratification and lack of available data. EO-AD is rarer than LO-AD, which contributed to the relative weakness of the statistical results. Second, this study did not include neuroimaging data from the LO-MCI subjects and asymptomatic normal controls. Thus, the EO-MCI, LO-MCI, and healthy subjects were not compared. Third, the brain images of EO-AD were not compared with those of LO-AD; thus, the characteristics were cited from other studies, which is also a limitation of this study. Finally, the EO-MCI group can be more heterogeneous than the LO-MCI group, including more patients with other comorbidities. Despite this limitations, the present study is significant in that it is a first attempt to identify the neuroimaging differences between EO-MCI and LO-MCI. Further research with increased number of EO subjects will be needed to identify the neuroimaging characteristics in EO-MCI.

ACKNOWLEDGEMENTS

This paper was supported by the research grant of Konkuk University in 2015.

Figure 1.

Left hippocampus radial distance (local shape analysis, FDR corrected). A: Bottom view of left hippocampus. B: Anterosuperior view of left hippocampus. FDR: false discovery rate.

Table 1.

Demographic and clinical characteristics of the subjects

	MCIcont (N=35)	ADConv (N=15)	Statistics t/χ²	p
Age (years)	63.81±3.19^*	63.64±3.01	t=0.149	0.883
Education (years)	9.10±2.55	8.91±2.25	t=0.276	0.785
Gender (%)			χ²=0.019	0.892
Men	15 (60.0)	7 (57.2)
Women	20 (40.0)	8 (42.8)
MMSE-KC	25.25±1.43	20.24±3.12	t=1.134	0.001^†
APOE ε4 carriers (%)	10 (25.8)	6 (40.0)	χ²=0.736	0.391

The statistics were analyzed using χ2 and t-test.

^* mean±standard deviation,

^† p<0.05.

MCI: mild cognitive impairment, MCIcont: MCI continued, ADconv: AD converted, MMSE-KC: Mini-Mental Status Examination in the Korean version of CERAD assessment, APOE: apoliphoprotein E

Table 2.

Changes in the hippocampal volume between the MCIcont and ADconv groups after 2 years

Hippocampus	MCIcont (N=35)				ADconv (N=15)
	APOE ε4 (-) (N=25)		APOE ε4 (+) (N=9)		APOE ε4 (-) (N=9)		APOE ε4 (+) (N=6)
	Baseline	2 years	Baseline	2 years	Baseline	2 years	Baseline	2 years
Left	2,338±343^*	2,231±344	2,318±357	2,204±354	2,329±364	21,94±352	2,317±374	2,181±363
Right	2,529±364	2,430±367	2,519±364	2,412±371	2,579±239	2,468±259	2,569±389	2,447±387

Age- and gender- adjusted repeated-measures ANOVA.

^* mean±standard deviation.

MCI: mild cognitive impairment, APOE: apolipoprotein E, MCIcont: MCI continued, ADconv: AD converted

Table 3.

Differences in atrophy rate between the left and right hippocampi (according to the APOE ε4 status)

Atrophy rate (%/year)	MCIcont (N=35)		ADconv (N=15)		p (2, 4)	p (3, 4)
Atrophy rate (%/year)	1; APOEε4 (-) (N=26)	2; APOEε4 (+) (N=9)	3; APOEε4 (-) (N=9)	4; APOEε4 (+) (N=6)	p (2, 4)	p (3, 4)
Left (% of changes)	2.29±0.89^*	2.46±0.87	2.64±1.02	2.93±1.18	0.005 (t=-2.91)^†	0.016 (t=-2.58)^†
Left (% of changes)	2.29±0.89^*	2.46±0.87	2.64±1.02	2.93±1.18	(tied p=0.003)^‡	(tied p=0.010)^‡
Right (% of changes)	1.96±0.58	2.12±0.56	2.15±0.63	2.34±0.99	0.060 (t=-1.97)	0.054 (t=-2.087)
Right (% of changes)	1.96±0.58	2.12±0.56	2.15±0.63	2.34±0.99	(tied p=0.081)^‡	(tied p=0.064)^‡

^* mean±standard deviation,

^† p<0.05,

^‡ for group comparisons, the unpaired t-test (two-tailed) was used together with Mann-Whitney U test.

MCI: mild cognitive impairment, APOE: apolipoprotein E, MCIcont: MCI continued, ADconv: AD converted

Table 4.

Effect of APOE ε4 on the atrophy rate of both hippocampi in the MCIcont and ADcov groups: results of the GLM analysis

	MCIcont (N=35)										ADconv (N=15)
	Left hippocampus					Right hippocampus					Left hippocampus					Right hippocampus
	Type III SS	df	Mean square	F	p	Type III SS	df	Mean square	F	p	Type III SS	df	Mean square	F	p	Type III SS	df	Mean square	F	p
Age	13.485	1	13.485	3.873	0.060	30.518	1	30.518	2.583	0.118	5.271	1	5.271	1.996	0.185	16.693	1	16.693	3.712	0.082
Gender	3.305	1	3.305	0.476	0.612	5.625	1	5.625	0.822	0.495	0.544	1	0.544	0.208	0.660	0.753	1	0.753	0.167	0.690
APOE ε4	19.258	1	19.258	5.532	0.025^*	45.139	1	45.139	3.82	0.059	34.281	1	34.28	12.978	0.001^†	29.188	1	29.188	11.05	0.007^†

^* p<0.05,

^† p<0.01.

APOE: apolipoprotein E, MCI: mild cognitive impairment, GLM: general linear model, SS: square sum, MCIcont: MCI continued, ADconv: AD converted

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